Cell Sorting
Multicolor fluorescence-activated cell sorting using the new FACSAria III flow cytometer from Becton Dickenson (BD). We offer services and consultancy regarding advanced cell sorting for private companies as well as hospitals and research institutes. We have a highly skilled staff of cell sorting specialists who can assist in the design of an optimal protocol for the specific project and subsequently perform the cell sorting.
In flow cytometry, a stream of small droplets each containing at the most a single cell is passed through an electronic detection device which is able to sort the drops according to the specific characteristics imposed by the single cell present in a given drop. Thus, a heterogeneous mixture of cells can be sorted into two or four containers, one cell at a time, typically using fluorescently labeled antibodies that recognize and bind to relevant cell surface proteins. In addition, sorting can be performed so that a specified number of cells can be applied directly into plates for subsequent biochemical analysis or culturing or onto slides for further microscopy analysis.
Immunologists have traditionally used flow cytometry for analysis but the technique is, in fact, highly relevant in many fields of cell science and clinical reserach. Flow cytometry is routinely used for diagnostic purposes in hospitals.
Our FACS ARIA III currently uses two lasers (488nm and 640nm) and is able to measure up to eight colors simultaneously.
The FACS ARIA III machine is handled by trained and highly experienced scientists and technicians in the field of immunology and mammalian cell biology.
Example: Isolation of human Treg cells (see figure below - click figure for larger image).
Figure 1: Sorting of human Treg cells (CD4+CD25hiCD127) and naive T cells (CD4+CD25-CD127hi). A cell population enriched for CD4 T cells was used for sorting. Dot-plots of pre sort and post sorts are shown (click figure for larger image).
For further information, please contact PhD Monika Gad by phone (+45 45160444) or email.