Cellular Assays

Bioneer has established a wide range of mammalian cell assays toassist our clients in the preclinical drug discovery phase or in the validation of medical devices. These assays currently comprise cytotoxicity assays, including apoptosis, and cell proliferation assays. In addition, we offer to develop novel functional assays in which specific drug mechanisms can be analysed.

Cytotoxic Assays
Bioneer offers general cell viability assays using LDH (lactate dehydrogenase), XTT and MTT assays for obtaining IC50 values. LDH is a stable enzyme, present in all cell types and which is rapidly released into the cell culture medium upon damage of the plasma membrane. MTT and XTT are compounds that are converted by mitochondrial dehydrogenases in live cells to a water insoluble purple formazan. These assays are typically done in 96-well formats and analysed in an ELISA Reader. These assays are applicable to both proliferating and non-proliferating cells.

Bioneer offers cell viability tests in any cell type relevant for the drug of interest.

We also offer cell cycle analysis by flow cytometry. This includes 7AAD- or PI-based assays in which apoptotic effects of drugs can be analysed. In addition, we can determine Relative Mitotic Index and the number of cells undergoing cell division in order to determine Cell Cycle Delay.

Finally, we have established comprehensive microscopic platforms to monitor morphological changes of cells in response to drug exposure.

Invasion assays
Bioneer offers cellular migration assays based on the AP48 screening platform. The platform can be used assessing the effect of anti-invasive drugs on relevant cell types.

Oxidative burst assay
Oxidative burst is the rapid production of reactive oxygen species (ROS) which are believed to contribute to the development of several major diseases. Bioneer offers ROS assays based on fluorescent probes and can evaluate the effect of drug candidates on the oxidative burst in relevant cell types.

For further information, please contact Group Leader, Mammalian Cell Technology; Christian Clausen by phone (+45 45160444) or email.