Fluorescence Applications

Our in situ hybridization technology platforms also allow fluorescence detection. Combining microRNA in situ hybridization with immunohistochemistry in a double fluorescence assay, for example to characterize the microRNA expressing cells with specific cell markers, is a possible second step in the histological microRNA expression analysis. The sample images shown on this page are examples of parallel fluorescence detection of a microRNA by in situ hybridization and subsequent immunohistochemical detection of a protein.

	miR-145 and cytokeratin. In the normal mammary gland, miR-145 is expressed in the myoepithelial cell layer. Here miR-145 is shown together with cytokeratin-positive (green) epithelial cells. Counterstain: DAPI.
	miR-205 and cytokeratin. In the normal mammary gland and in ductal carcinoma in situ (DCIS), miR-205 is expressed in the myoepithelial cell layer. Here miR-205 (red) is shown together with cytokeratin-positive (green) epithelial tumor cells. Counterstain: DAPI.
	miR-205 and smooth muscle alpha-actin. In the normal mammary gland, miR-205 is expressed in the myoepithelial cell layer. Here miR-205 (green) is shown together with smooth muscle alpha-actin (red) in myoepithelial cells. Counterstain: DAPI.