Fluorescence Applications

Our in situ hybridization technology platforms also allow fluorescence detection on a limited number of samples. Combining microRNA in situ hybridization with immunohistochemistry in a double fluorescence assay, for example to characterize the microRNA expressing cells with specific cell markers, is a possible second step in the histological microRNA expression analysis.

miR-205 and CD31

In the normal mammary gland, miR-205 is expressed in the myoepithelial cell layer. CD31 (PECAM1) is a common marker of endothelial cells. In this image, miR-205 (green) is seen in a ductal structure and CD31 (red) in vessels located in the surrounding mesenchymal tissue. Counterstain: DAPI.